DNA Pronuclear Microinjection

Direct microinjection of DNA into the pronucleus of a mouse zygote has been until recently the method most extensively used in the production of transgenic mice. If the foreign DNA integrates into the mouse chromosomal DNA at the one-cell stage, the animal will contain the injected DNA in every cell, including those of the germ line. If, however, integration occurs later, the resulting animals will be mosaic for the presence of the injected DNA.

Upon receipt of the DNA construct, proven by documentation to be of high quality and suitable for microinjection, we will proceed to injecting.

Fees are specific to the requirements of the individual project. Services offered are flexible and projects can be customized to meet individual needs. For project-based pricing please contact us at mapsinfo@cmmt.ubc.ca