Bioanalyzer

Bioanalyzer Core Facility

The CMMT / BCCHR Bioanalyzer Core Facility provides researchers at CMMT, BCCH Research Institute and within the Greater Vancouver community access to the microfluidics based functionality of the Bioanalyzer from Agilent Technologies (Agilent 2100 Bioanalyzer System).

The Bioanalyzer system enables rapid, accurate and reproducible analysis of the quality and composition of RNA, DNA and protein samples with exceptionally small sample quantities and with many advantages over gel electrophoresis methods. It is the standard method utilized for qualitative and quantitative analysis of RNA samples prior to analysis on microarray systems.

Since its inception, the facility has analyzed over 15,000 samples for 60 UBC research groups at the CMMT, BCCHR, Michael Smith Labs, iCapture, iCORD, Microbiology, and Botany, as well several industry research groups.

Overview of technology

img_bioanalyzer_platform
The Bioanalyzer Core Facility offers you access to the advanced functionality of the Agilent 2100 bioanalyzer. The Agilent 2100 bioanalyzer uses lab-on-a-chip technology to provide improved analysis of RNA, DNA, and proteins through:

 

  • Automation for improved accuracy and reproducibility;
  • Rapid visualization of sample quality and quantity;
  • High sensitivity with minimal sample quantities;
  • Significant time savings—as fast as same day service;
  • Easy comparison or sharing of digital sample data;
  • Simple, robust protocols and reagents; and
  • Reduced use and waste of hazardous chemicals;

Additional information on the Agilent 2100 BioAnalyzer platform can be found on the Agilent website (link). Click here to view our summary of the principles of nucleic acid and protein analysis on the 2100.

Services available from the facility

The facility currently keeps the following LabChip kits in stock typically providing same or next day service.

KitSamples analyzedSamples per chipInfo
RNA Nano LabChip KitRNA 5-500 ng/ul12Info

Guide
RNA Pico LabChip KitRNA
200-5,000 pg/ul
11Info

Guide

The facility is able to provide analyses utilizing the following LabChip kits, though they are not kept in stock. Please contact us for further information.

KitSamples analyzedSamples per chipInfo
Small RNA LabChip KitSmall RNA, miRNA
6 to 150 nt
50 to 2000 pg/ul
11Info

Guide
DNA LabChip KitsDNA or siRNA
25bp-12kb
12Info

Guide
High Sensitivity DNA
LabChip Kits
DNA
50bp-7kb
5-500 pg/ul
11Info

Guide
Protein LabChip KitsProtein
5-230 kDa
10Info

Guide

Sample Submission

For information on sample preparation and to submit samples please download the sample submission form (PDF / WORD)

Results Analysis

Bioanalyzer results will be sent via email and can be completed as quickly as the same day as sample submission. Results are viewed and analyzed and printed using the free Agilent 2100 expert software (this is the same analysis software used by the facility).

Extensive online help is included on how to use the software. Please click on RNA or DNA to see data analysis examples and instructions from the online help.

If you do not have a system that can run the Agilent 2100 expert software, please contact us for assistance with other options.

Pricing

For CMMT and CFRI labs, the cost per sample for the RNA Nano analysis is approximately $8.60. A charge of approx. $6.95 will be applied for each un-used well on a chip. These empty well charges are minimized by submitting samples in multiples of the number of wells on a chip (see LabChip Kit table above) or when samples from other users are run on the same chip. Please enquire for pricing if your group is from outside of the CMMT/CFRI or for other kits.

If you are not sure if the Agilent 2100 technology is suitable for your research needs, please contact us about running a couple of your samples for free.

Monthly billing for services is either by Journal Voucher for UBC researchers or invoice.

Contact Information

For additional information and to submit samples please contact:

Ms. Liz Hui
(604) 875-3814
2100@cmmt.ubc.ca

Mailing Address:

CMMT/CFRI Bioanalyzer Core Facility
Attention: Ms. Liz Hui
The Centre for Molecular Medicine and Therapeutics
950 West 28th Avenue, Room A3-193
Vancouver, BC
V5Z 4H4 Canada

For further information on CMMT Core Facilities, contact:

Michael Hockertz
hockertz@cmmt.ubc.ca
(604) 875-3816