Bioanalyzer Core Facility
Bioanalyzer Core FacilityThe CMMT and CFRI Bioanalyzer Core Facility was established in 2003 with the assistance of an equipment grant from CFRI. This core facility provides researchers at CMMT, CFRI and within the Greater Vancouver community access to the state-of-the-art microfluidics-based functionality of the Bioanalyzer from Agilent Technologies. The 2100 Bioanalyzer system enables rapid, accurate and reproducible analysis of the quality and composition of RNA, DNA and protein samples with exceptionally small sample quantities and with many advantages over gel electrophoresis methods. It is the standard method utilized for qualitative and quantitative analysis of RNA samples prior to analysis on microarray systems. Since its inception, the facility has analyzed over 6,500 samples for 32 UBC research groups at the CMMT, CFRI, Michael Smith Labs, iCapture, iCORD, Microbiology, and Botany, as well several industry research groups. The CMMT/CFRI BioAnalyzer Core Facility provides researchers at the Children's and Women's Hospital site and within the Greater Vancouver community access to the state-of-the-art microfluidics-based functionality of the Agilent 2100 bioanalyzer system from Agilent Technologies. 
This system enables rapid, accurate and reproducible analysis of the quality and composition of RNA, DNA and protein samples with exceptionally small sample quantities and with many advantages over gel electrophoresis methods. The 2100 bioanalyzer system is the de facto standard for qualitative and quantitative analysis of RNA samples prior to analysis on microarray systems. Overview of technology
The CMMT/CFRI BioAnalyzer Core Facility offers you access to the advanced functionality of the Agilent 2100 bioanalyzer. The Agilent 2100 bioanalyzer uses lab-on-a-chip technology to provide improved analysis of RNA, DNA, and proteins through:
For more information...Additional information on the Agilent 2100 BioAnalyzer platform can be found on the Agilent website (link). Click here to download the Agilent brochure (1.10 MB, PDF). Click here to view our summary of the principles of nucleic acid and protein analysis analysis on the 2100. Services Available from the FacilityThe facility currently provides analyses utilizing the following LabChip kits.
Sample submissionFor information on sample preparation and to submit samples, please download the sample submission form in Microsoft Word format (85 KB) or in Adobe Acrobat (PDF) (131 KB) format. Results analysisBioAnalyzer results will usually be sent via email and can be completed as quickly as the same day as sample submission. Results are viewed and analyzed and printed using the free Agilent 2100 expert software (this is the same analysis software used by the facility). Minimum requirements are an 1.5 Ghz Pentium 4 with 512 MB RAM with either Windows 2000 Service Pack 4 (or higher) or Windows XP Service Pack 2 (or higher). Please contact the Facility for a copy of the software (Version B.02.02) as the version available for download at Agilent (Version B.02.03) may not be compatible. Extensive online help is included on how to use the software. Please click on RNA, DNA, or protein to see data analysis examples and instructions from the online help. If you do not have a system that can run the Agilent 2100 expert software, please contact us for assistance with other options. PricingThe cost per sample is approximately $3.75 to $6.50 depending primarily on the type of sample analyzed. A materials cost of $2 to $4 will be charged for each un-used well on a chip. These empty well charges are minimized by submitting samples in multiples of the number of wells on a chip (see LabChip Kit table above) or when samples from other users are run on the same chip. If you are not sure if the Agilent 2100 technology is suitable for your research needs, please contact us about running a couple of your samples for free. Monthly billing for services is either by Journal Voucher for UBC researchers or invoice. CONTACT FORM For your convenience, we have set up a service contact form. Please feel free to use this form to contact us regarding our laboratory services. CONTACT INFORMATION For additional information and to arrange to submit samples please contact:
CMMT/CFRI Bioanalyzer Core Facility
Attention: Ms. Xiaohua Han The Centre for Molecular Medicine and Therapeutics 950 West 28th Avenue, Room A3-193 Vancouver, BC V5Z 4H4 Canada Direct: +1 (604) 875-3814 Fax: +1 (604) 875-2494 For more information please contact Michael Hockertz.
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