Bioanalyzer Core Facility

Bioanalyzer Core Facility

The CMMT and CFRI Bioanalyzer Core Facility was established in 2003 with the assistance of an equipment grant from CFRI. This core facility provides researchers at CMMT, CFRI and within the Greater Vancouver community access to the state-of-the-art microfluidics-based functionality of the Bioanalyzer from Agilent Technologies (Agilent 2100 Bioanalyzer System).

The 2100 Bioanalyzer system enables rapid, accurate and reproducible analysis of the quality and composition of RNA, DNA and protein samples with exceptionally small sample quantities and with many advantages over gel electrophoresis methods. It is the standard method utilized for qualitative and quantitative analysis of RNA samples prior to analysis on microarray systems.

Since its inception, the facility has analyzed over 11,000 samples for 45 UBC research groups at the CMMT, CFRI, Michael Smith Labs, iCapture, iCORD, Microbiology, and Botany, as well several industry research groups.

Agilent 2100 BioAnalyzer platform

Overview of technology

RNA Pico Results

The CMMT/CFRI BioAnalyzer Core Facility offers you access to the advanced functionality of the Agilent 2100 bioanalyzer. The Agilent 2100 bioanalyzer uses lab-on-a-chip technology to provide improved analysis of RNA, DNA, and proteins through:

  • Automation for improved accuracy and reproducibility;

  • Rapid visualisation of sample quality and quantity;

  • High sensitivity with minimal sample quantities;

  • Significant time savings—up to 12 samples in 30 minutes;

  • Easy comparison or sharing of digital sample data;

  • Simple, robust protocols and reagents; and

  • Reduced use and waste of hazardous chemicals;


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For more information...

Additional information on the Agilent 2100 BioAnalyzer platform can be found on the Agilent website (link). Click here to download the Agilent brochure (1.10 MB, PDF). Click here to view our summary of the principles of nucleic acid and protein analysis analysis on the 2100.


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Services Available from the Facility

The facility currently keeps the following LabChip kits in stock typically providing same or next day service.

Kit Samples
analyzed
Samples
per chip
Info
RNA Nano
LabChip Kit
RNA
5-500 ng/ul
12 Info
Guide



The facility is able to provide analyses utilizing the following LabChip kits, though they are not kept in stock. Please contact us for further information.

Kit Samples
analyzed
Samples
per chip
Info
Small RNA
LabChip Kit
Small RNA, miRNA
6 to 150 nt
50 to 2000 pg/ul
11 Info
Guide
RNA Pico
LabChip Kit
RNA
200-5,000 pg/ul
11 Info
Guide
DNA
LabChip Kits
DNA or siRNA
25bp-12kb
12 Info
DNA 1000 Guide
DNA 7500-12K Guide
High Sensitivity
DNA
LabChip Kits
DNA
50bp-7kb
5-500 pg/ul
11 Info
Guide
Protein
LabChip Kits
Protein
5-230 kDa
10 Info
P80 Guide
P230 Guide


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Sample submission

For information on sample preparation and to submit samples, please download the sample submission form in Microsoft Word format (85 KB) or in Adobe Acrobat (PDF) (107 KB) format.


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Results analysis

BioAnalyzer results will usually be sent via email and can be completed as quickly as the same day as sample submission. Results are viewed and analyzed and printed using the free Agilent 2100 expert software (this is the same analysis software used by the facility).

Extensive online help is included on how to use the software. Please click on RNA, DNA, or protein to see data analysis examples and instructions from the online help.

If you do not have a system that can run the Agilent 2100 expert software, please contact us for assistance with other options.


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Pricing

For CMMT and CFRI labs, the cost per sample for the RNA Nano analysis is approximately $8.60. A charge of approx. $6.95 will be applied for each un-used well on a chip. These empty well charges are minimized by submitting samples in multiples of the number of wells on a chip (see LabChip Kit table above) or when samples from other users are run on the same chip. Please enquire for pricing if your group is from outside of the CMMT/CFRI or for other kits.

If you are not sure if the Agilent 2100 technology is suitable for your research needs, please contact us about running a couple of your samples for free.

Monthly billing for services is either by Journal Voucher for UBC researchers or invoice.


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doc_cmmt_2100_submissions.doc75.5 KB
pdf_cmmt_2100_submissions.pdf24.97 KB

CONTACT FORM
For your convenience, we have set up a service contact form. Please feel free to use this form to contact us regarding our laboratory services.

CONTACT INFORMATION
For additional information and to arrange to submit samples please contact:
Ms. Liz Hui
+1 (604) 875-3814
2100@cmmt.ubc.ca
www.cmmt.ubc.ca/2100
Ms. Xiaohua Han
+1 (604) 875-3814
2100@cmmt.ubc.ca
www.cmmt.ubc.ca/2100
CMMT/CFRI Bioanalyzer Core Facility
Attention: Ms. Liz Hui
The Centre for Molecular Medicine and Therapeutics
950 West 28th Avenue, Room A3-193
Vancouver, BC
V5Z 4H4 Canada
Direct: +1 (604) 875-3814
Fax: +1 (604) 875-2494

For more information please contact Michael Hockertz.
Director of Core Facilities
+1 (604) 875-3816
hockertz@cmmt.ubc.ca