Cryopreservation Services

MAPS is pleased to offer a secure cryopreservation program to CMMT and CFRI researchers currently maintaining mouse colonies within the CMMT Mouse Facility. Sperm cryopreservation offers an efficient, cost effective method of strain preservation and is currently the preferred technique utilized by our barrier facility users.

Preserving rare and unique transgenic strains, preventing phenotype loss and reducing genetic drift, as well as protection against breeding cessation are some reasons to cryopreserve your mouse strains. Cryopreservation is more cost effective than maintaining breeding colonies that are not currently involved in any studies, reduces the amount of technician time spent on colony management, and frees up valuable space within the animal facility. Cryopreservation also reduces the risk of losing your precious mouse strains to natural disasters such as earthquakes and floods, disease outbreaks or contamination within the facility, and other unpredictable situations.

ANIMAL REQUIREMENTS

Sperm Cryopreservation

  • 2 males age 10-16 weeks.
  • Males that have not been bred
  • 5 donor females (background strain) are required for IVF to assess the fertilization rate of the cryopreserved sperm.

SPERM CRYOPRESERVATION

  • 20 straws frozen per strain.
  • ~10 recoveries to live born pups if researcher requests resurrection of the strain.
  • All cryopreserved sperm is recovered via in-vitro fertilization to assess the sperm viability.

Cryopreserved sperm with a fertilization rate of above 20% is considered to be successfully frozen. However, there is always a chance that strains which exhibit good fertilization rates are not capable of generating live born pups. Because of this investigators can choose to have the embryos resulting from IVF, cultured to blasts, and if desired transferred into pseudo-pregnant recipients to generate live born pups as a guarantee that their strain will be recoverable. Researchers are asked to maintain active breeding colonies until the cryopreservation of their strain has been proven successful.

FEES

Standard sperm cryopreservation projects include one year of liquid nitrogen storage inside the barrier facility and the cost of IVF fertilization to test the viability of the cryopreserved sperm. Strain recovery to live born pups requires an additional fee to cover cage per diems and the extra surgical procedures. Any cryopreservation projects with males that do not meet the ideal criteria above may require a special fee structure. MAPS also offers strain rescue and in-vitro fertilization (IVF) services. Please contact us at mapsinfo@cmmt.ubc.ca